Improved isolation of Salmonellae from naturally contaminated meat products by using Rappaport-Vassiliadis enrichment broth.
نویسندگان
چکیده
A total of 454 specimens of meat products were examined for salmonellae by using five procedures of enrichment. The use of a selective motility medium, inoculated from enrichment in Muller-Kauffmann broth, resulted in an increase in the number of positive specimens. However, simple enrichment in Rappaport-Vassiliadis broth, after preenrichment, was more sensitive and specific for recovering salmonellae than the selective motility medium-Muller-Kauffmann broth method.
منابع مشابه
Comparison of Six Culture Methods for Salmonella Isolation from Poultry Fecal Samples
Abstract Background and Objective: Salmonellosis is one of the most important food-borne bacterial zoonotic diseases worldwide, and poultry and its products are the major sources for salmonella transmission to human. Isolation of Salmonellaenterica from poultry needs bacteriologic enrichment and selected cultures of fecal samples. In this study, different culture methods for the isolation of s...
متن کاملA Recommended Method for Detecting Salmonellae in Composted Biosolids
It has been found in Australia and in the United States that composting does not always result in the complete removal of salmonellae from biosolids. It is therefore likely that monitoring of composted biosolids for salmonellae will be required in Australia to ensure the safety ofbiosolids products. At present rapid methods of detection such as PCR and ELISA are not sufficiently developed to mo...
متن کاملEffect of the enrichment medium on the detection and diversity of Salmonella from porcine duodenal content.
This study assesses the effect of the enrichment medium used on the isolation of Salmonella from the duodenal content of naturally infected slaughter pigs. At six slaughterhouses, the duodenum was collected from 458 randomly chosen pigs and examined in the laboratory. Three semi-solid enrichment media (modified semi-solid Rappaport-Vassiliadis medium [MSRV], diagnostic semi-solid Salmonella med...
متن کاملMonitoring of the dissemination of Salmonella in the chicken Frankfurt-sausage production line of a sausage factory in the state of São Paulo, Brazil.
Poultry meat and its derivatives are among the foodstuffs considered by environmental health authorities to present the highest risks to the public. A total of 185 samples were collected in five monthly batches, from different processing stages in a sausage plant that uses mechanically-deboned chicken meat (MDCM), and tested for the presence of Salmonella. Enrichment was carried out in both Kau...
متن کاملبررسی انواع محیطهای غنی کننده، انتخابی و افتراقی درجداسازی و شناسایی سالمونلا در مبتلایان به اسهال
Background and Aim: Salmonellosis is a gastroenteritis caused by different serotypes of Salmonella and is the most common type of food poisoning in the world. The purpose of this research study is to optimize the conventional method for the isolation of Salmonella SPP from the diarrheic specimens of children. Materials and Methods: Stool specimens were obtained from one hundred patients admitt...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 42 4 شماره
صفحات -
تاریخ انتشار 1981